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Siberian Journal of Forest Science

2019 year, number 3

THE STUDY OF ANTI-MICROBIAL PROPERTIES OF EXTRACTIVE SUBSTANCES OF CONIFERS

V. A. Senashova, G. V. Permyakova, I. D. Grodnitskaya, N. V. Pashenova, S. R. Loskutov
V. N. Sukachev Institute of Forest, Russian Academy of Science, Siberian Branch, Krasnoyarsk, Russian Federation
Keywords: экстракция коры хвойных, концентрированные экстракты, фенольные соединения, антимикробные свойства, conifer bark extraction, concentrated extracts, phenolic compounds, anti-microbial properties

Abstract

The problem of wood waste utilization is of great importance. In connection with the outbreaks of microorganisms resistant to most of the known antibiotics researchers are increasingly interested in natural objects that can serve as raw materials for producing medicines, including those with antimicrobial activity. Bark of conifers is a source of many biologically active substances. From the pharmacological and therapeutic points of view, phenolic compounds of bark are most interesting. The extraction of the bark of conifers with monoethanolamine provides a product with an increased proportion of phenolic compounds in comparison with other known methods. The antibacterial activity of the extracts and their solutions obtained by the abovementioned method from the bark of conifers of Siberian larch Larix sibirica L. , Scotch pine Pinus sylvestris L., and Siberian fir Abies sibirica L. was studied. We used the museum strains of sanitary-indicative opportunistic bacteria such as Klebsiella pneumoniae (Schroeter) Trevisan Т 904, Escherichia coli Migula ATCC 39/21141, Staphylococcus aureus Rosenbach АТСС 25922, Proteus vulgaris Hauser MX 19 и Micrococcus luteus (Schroeter) Cohn emend. Wieser et al. ATCC 9341 as test objects It was found that the extract of Scotch pine demonstrated greater inhibitory activity while fir bark extract had the lowest bacteriostatic properties. The diluted extracts of larch and Scotch pine bark were less effective against the tested bacteria, and the diluted extracts of fir bark had no antibacterial effect. The obtained data are promising for further research of the development of antibiotic / disinfecting substances based on conifer bark extracts.