Model of the Vibrio Сholerae Biofilm as a Mchanism of its Existence in Surface Water Reservoirs
E. S. KULIKALOVA, S. G. SAPPO, L. Ya. URBANOVICH, E. Yu. MARKOV, L. V. MIRONOVA, S. V. BALAKHONOV
Irkutsk Antiplague Research Institute of Siberia and Far East of Rospotrebnadzor, 664047, Irkutsk, Trilisser str., 78
Keywords: Vibrio cholerae eltor, biofilm, structure, genotype, chitin
Abstract
Vibrio cholerae eltor strains with different epidemic importance isolated from river water in Vladivostok during the cholera outbreak (1999) and in Irkutsk during the disease-free period (2005) were used in the experiment. Biofilm structure consisting of a peripheral part, bundles, polysaccharide matrix, channels and polymorphic vibrios is presented using light and luminescent microscopy. Metachromatic pink colouring of the matrix (crystal-violet, toluidine blue) or fluorescent reddish-orange colour (acridine orange) indicate that acid mucopolysaccharides (glucosamineglucans) are present in its structure. Biofilm of a toxigenic strain is formed much later than the non-toxigenic strain biofilm, and the elements making its structure are more apparent. Viability of V. cholerae cells during the whole experiment (90 days) and preservation of initial pathogenic potential indicate high adaptive properties of V. cholerae eltor that provide its survival and reproduction in surface water reservoirs under favourable ecological conditions (optimum temperature, existence of a chitin-containing substratum, etc.).
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