Ecological and Geographic variability of Hedysarum Theinum and Characteristics of its Propagation in Vitro
A. A. ERST, T. V. ZHELEZNICHENKO, T. I. NOVIKOVA, O. V. DOROGINA, E. V. BANAEV
FSIS Central Siberian Botanical Garden SB RAS, 630090, Novosibirsk, Zolotodolinskaya st., 101
Keywords: Hedysarum theinum Krasnob, cenopopulation, intrapopulation variability, productivity of aboveground and underground biomass, electrophoretic spectra, polypeptides of seeds, microclonal propagation
Abstract
The link between productivity of aboveground and underground biomass and variability of electrophoretic spectra of seeds polypeptides was established on the basis of the analysis of 6 populations of a valuable medicinal species – Hedysarum theinum Krasnob. The protocols for microclonal propagation of perspective samples were developed. The most efficient multiplication was achieved in MS medium supplemented with 5 µM BAP, 200 mg/l of glutathione and 200 mg/l of casein hydrolyzate. The shoots were successfully rooted on half strength MS basal medium supplemented with 7 µM NAA.
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