STUDY OF DNA DAMAGE BY NEW NORMOTHYMIC MEDICINAL ON THE BASIS OF THE COMPLEX OF LITHIUM CITRATE, POLYMETHYLSYLOXANE AND ALUMINUM OXIDE BY DNA COMET ASSAY IN VIVO
Vladimir Iosifovich KONENKOV1, Lyubov’ Nikiforovna RACHKOVSKAYA1, Andrey Yur’evich LETYAGIN1, Tat’yana Gennad’evna BOROVSKAYA2, Anna Veniaminovna SHURLYGINA1, Margarita Vladimirovna ROBINSON1, Maksim Aleksandrovich KOROLEV1, Anastasiya Anatol’evna KOTLYAROVA1, Tat’yana Viktorovna POPOVA1, Edmund Edmundovich RACHKOVSKIY1, Anna Vladimirovna VYCHUZHANINA2, Valeriya Aleksandrovna MASHANOVA2
1Research Institute of Clinical and Experimental Lymрhology - Branch of the Institute of Cytology and Genetics of SB RAS
2Gol’dberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center of RAS
Keywords: лекарственное средство, комплекс лития цитрата, полиметилсилоксана и оксида алюминия, повреждение ДНК, метод ДНК-комет, medicinal product, a complex of lithium citrate, polymethylsiloxane and aluminum oxide, DNA damage, DNA comet asay
Abstract
The aim of the study was to study the effect of the normotimic drug based on a complex of lithium citrate, polymethylsiloxane and alumina on DNA damage level (DNA comet test) in vivo by alkaline gel electrophoresis. It was shown that at 3 and 18 hours after the drug administration at the highest dose (2000 mg/kg) and therapeutic dose (400 mg/kg), the percentage of DNA in the tail in the cells of the bone marrow does not differ from the negative controls. That may indicate absence of possible carcinogenic action of the lithium-containing drug. On the other hand, the amount of DNA damage in other organs (kidney, liver, large intestine) increased with an exposure time of 3 hours after administration of the drug in a toxic dose. Although, the degree of this effect expression was not high. When the dose was reduced (400 mg/kg) and the duration of the experiment was increased (up to 18 hours), this effect was not detected. That indicates the ability of the drug to enhance DNA damage repair. Given that lithium preparations have a pronounced toxic effect on kidneys, gastrointestinal tract, liver, the increase in the percentage of DNA in the tail in these organs can be a consequence of the cytostatic action of the drug.
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