CORRELATION BETWEEN CYTOKINE CONTENT IN LYMPH OF THORACIC LYMPH DUCT AND MESENTERIC LYMPH NODE STRUCTURAL TRANSFORMATIONS IN EXPERIMENTAL MAMMARY TUMOR AND CHEMOTHERAPY
Oleg Vasilievich KAZAKOV, Tatyana Vladimirovna RAYTER, Alexandr Fedorovich POVESHCHENKO, Nikolay Borisovich ORLOV, Olga Vladimirovna POVESHCHENKO, Alexey Vasilievich KABAKOV, Alexandr Petrovich LYKOV, Irina Innokentyevna KIM, Nataliya Anatolyevna BONDARENKO, Dmitriy Nikolaevich STRUNKIN, Vladimir Iosifovich KONENKOV
Research Institute of
Clinical and Experimental Lymphology - Branch of Federal Research Center
Institute of Cytology and Genetics of SB RAS
Keywords: лимфатические узлы, молочная железа, опухоль, терапевтические мероприятия, профилактическое лечение, lymph nodes, mammary gland, tumor, therapeutic measures, preventive treatment
Abstract
The aim of the study was to
fulfill correlation analysis of morphometry of the mesenteric lymph
nodes and the concentration of cytokines in the lymph of the thoracic
duct in breast cancer induced by intramammary administration of
N-methyl-N-nitrosourea, chemotherapy according to the CMF scheme
(cyclophosphamide, methotrexate, 5-fluorouracil). The results of the
study. At breast cancer revealed positive correlation: in the
germinative centers and medullary cords of cytokine IL-5 with
mitotically dividing cells, chemokines MIP-1α with average lymphocytes,
in the germinative centers of immunoblasts with cytokine GRO/KC, in the
paracortical zone chemokine MCP-1 with macrophages, reticular cells with
IL-6 and M-CSF, in the medullary sinuses chemokine GRO/KC with small
lymphocytes and mature plasma cells (number which decreases). All this
may indicate the activity of the local immune response in the lymph
nodes aimed on the antitumor protection. After chemotherapy of breast
cancer, compared with breast cancer without treatment, revealed positive
relationship, which may indicate increased immunomodulatory and
antitumor actions of cytokines: correlation of interferon IFNγ with
small lymphocytes (number which increased) and macrophages in the
germinative centers and mitotically dividing cells in the medullary
cords, correlation in the germinative centers of immunoblasts with
MIP-1α and increased of number small lymphocytes in T-dependent zone
lymph nodes, correlation in medullary cords of interleukin IL-17 with
mature plasma cells (number which increased) , correlation of
interleukin IL-18 with mature plasma cells in medullary sinuses.
Conclusion. Study of the correlation of the concentration of cytokines
in the lymph of the thoracic duct with structural changes in the
mesenteric lymph nodes revealed dependencies aimed at increasing the
immunomodulating and antitumor effects of cytokines
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