The safety of the use of the conditioned medium obtained by directed osteogenic induction of rat bone marrow mesenchymal stem cells
Lyubov’ A. Pokrovskaya1, Evgeniy Yu. Sherstoboev1,2, Sergey V. Nadezhdin3, Marina G. Danilets4, Evgeniya S. Trofimova4, Anastasiya A. Ligacheva4, Aleksey A. Churin4, Tat’yana Yu. Dubskaya4
1National Research Tomsk State University, Tomsk, Russia
pokrovskayal@ect-center.com
2Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center of RAS
sherstoboev_eu@pharmso.ru
3Belgorod National Research University, Belgorod, Russia
sergey_nadezhdin@yahoo.com
4Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center of RAS, Tomsk, Russia
danilets_mg@pharmso.ru
Keywords: мезенхимальные стволовые клетки костного мозга крыс, кондиционная среда, цитотоксичность, подострая токсичность, крысы SD, rat bone marrow mesenchymal stem cells, conditioned medium, cytotoxicity, subacute toxicity, SD rats
Abstract
The purpose of the study was to evaluate in vitro cytotoxicity and in vivo sub-acute toxicity of conditioned medium obtained from rat bone marrow-derived mesenchymal stem cells directed to osteogenic differentiation. Material and methods. Conditioned medium was obtained by culturing rat bone marrow-derived MSCs induced under osteogenic condition. Results and discussion. Conditioned medium from rat bone marrow-derived MSCs was shown to have no significant cytotoxic effect on Ehrlich adenocarcinoma cell culture and in vitro expanded peripheral blood mononuclear cells from healthy donors. The use of MSC-CM did not have a significant effect on the state of experimental SD rats, the total body weight and growth rate of animals. A pathomorphologic study revealed no any abnormalities associated with MSC-CM injection. Conclusion. The use of conditioned medium from rat bone marrow-derived mesenchymal stem cells stimulated under osteogenic condition was found to be safe for both in vitro studies (with respect to Ehrlich adenocarcinoma cell line and peripheral blood mononuclear cells from healthy donors) and in vivo studies (injection of MSC-CM to animals).
|
