THE POSSIBILITY OF USING ISSR-MARKERSFOR DETECTION OF GENETIC DIFFERENTIATION OF POPULATIONS OF RARE SPECIES OF ASTRAGALUS SERICEOCANUS (FABACEAE)
I.Yu. Selyutina, E.S. Konichenko, O.V. Dorogina
Central Siberian Botanical Garden, SB RAS, 630090, Novosibirsk, Zolotodolinskaya str., 101
selyutina.inessa@mail.ru
Keywords: Fabaceae, Astragalus sericeocanus, генетическая изменчивость, ISSR, редкий вид, охрана рас- тений, оз. Байкал, Fabaceae, Astragalus sericeocanus, genetic diversity, ISSR, rare species, plant conservation, Lake Baikal.ВВЕДЕНИЕ
Abstract
Inter-simple sequence repeats (ISSR) markers of genomic DNA were used to assess the genetic diversity in six popu- lations of Astragalus sericeocanus - rare endemic species to the shore of Lake Baikal. Five primers amplified 91 bands with 86 (94.1 %) being polymorphic. A low level of genetic similarity among population Turka and other populations was detected, based on the Nei’s distance (0.145). The low genetic connectivity was found among population Turka and other populations is an evidence of enough long-term insulation this population on the eastern coast of Lake Baikal. Based on these results the conservation strategy of Astragalus sericeocanus was proposed.
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