Th e effi cient systems of in vitro establishment and direct shoot regeneration from floral explants of frost-resistant genotypes of Rhododendron L. genus promising for landscaping and breeding (semi-evergreen species R. sichotense Pojark. and evergreen cultivar ‘Pohjola′s Daughter’) were developed. The isolated pistil with pedicle was used as a floral explant. The effects of various thidiazuron (TDZ) concentrations and TDZ combinations with 2-isopentiladenin (2-iP) and indolyl-3-butyric acid (IBA) as well as with zeatin were tested. Th e regeneration occurred directly from pedicle tissues. The study revealed the genotypic differences between morphogenic responses of ‘Pohjola′s Daughter’ and R. sichotense fl oral explants. To induce the regeneration from ‘Pohjola′s Daughter’ floral explants, the optimum medium was Andersen’s medium (AM) supplemented with 2.5 μM TDZ in combination with 73.8 μM 2-iP and 15.0 μM IBA whereas the presence of 1.0 μM TDZ was suffi cient for R. sichotense regeneration. The maximum of shoots per explant obtained when explants culturing on optimum composition of AM amounted to 26.21 ± 5.26 for ‘Pohjola′s Daughter’ and 17.40 ± 3.07 for R. sichotense . Th e developed clonal micropropagation protocols based on the direct shoot regeneration from floral explants R. sichotense and ‘Pohjola′s Daughter’ can be used for genetic resources conservation, breeding program development and commercial large-scale propagation.
Th e effective technologies of in vitro establishment and micropropagation of three cultivated strawberry genotypes ( Fragaria × ananassa Duch.) acclimatizated for Siberian region (‘Sunny Glade’, ‘June Smile’ and the selected hybrid of 5-90-21) have been developed using the stolon apical meristems as explants. The sterilization of explants was optimized by application of 0.2 % silver nitrate solution as a sterilizing agent. Th e effects of various 6-benzylaminopurine (BAP) concentrations (0.25-2.0 mg/l) on in vitro explant regeneration potential on Murashige- Skoog (MS) and Gamborg-Eveleigh (B5) media were studied. The best medium variant to initiate the organogenesis from ‘June Smile’ cultivar was MC supplemented with 1.0 mg/l BAP, whereas for 5-90-21 hybrid and ‘Sunny Glade’ cultivar it was B5 with the same BAP concentration. Subsequent cultivation of the developed microshoots on media for micropropagation has shown the effectiveness of B5 medium supplemented with 0.75 mg/l BAP for all genotypes: maximum number of axillary microshoots per explant was amounted to 4.90 ± 0.35 for hybrid 5-9021, 7.16 ± 0.33 - for ‘Sunny Glade’ and 5.93 ± 0.39 - for ‘June Smile’. The obtained results can be used for development of production system of a healthy planting strawberry material through biotechnological approaches.
In A.A. Achimova′s book “Plants of Altai mountains in customs and traditions of the Altains” information is collected about the plants use as a totem, in ceremonies such as wedding or funeral, in birth and upbringing of a child, in the traditional kitchen, in medicine, in hunting. It is noted that although plants are still used by Altai people their importance in all activities is reduced. Number of people with knowledge of plants consumed in everyday life, is reduced. For each referred plant local names are given along with Russian and scientifi c Latin names, ethnic groups using these plants are given. Book contains characteristics of 141 species of plants from 40 families of natural fl ora. The work is illustrated with photographs presenting some points on plant cult in Altai. Book is a monument to ethnoculture, allowing preserving information on passing traditional values.
The present paper considers a species composition of foliar pathogenic micromycetes of Salix L. introductions growing in introduction centers and landscape plantations of five Siberian cities. Nine pathogens are revealed, two of them are given to Siberia for the first time. Morphology, pattern of fungi biology and pathogenesis are described. Host plants, general distribution of phytopathogens and occurrence in the model area are indicated. Intraspecific pathogen resistance is established for certain Salix L. introductions.
New data about some genus Linum L. species in flora of Siberia are presents. In Siberia, there are 10 species of this genus, belonging to the 2 subgenera ( Linum and Cathartolinum (Rchb.) Svetlova) and 3 sections ( Stellerolinum Juz. ex Prob., Linum and Adenolinum (Rchb.) Juz.). Th e section Adenolinum comprises 7 species ( L. perenne L., 2 subspecies L. komarovii Juz., L. altaicum Ledeb. ex Juz., L. amurense Alef., L. baicalense Juz., L. violascens Bunge and L. pallescens Bunge) classifi ed into 3 subsections ( Longiunguiculata Svetlova, Amurense Svetlova and Breviun guiculata Svetlova). According to its morphological characteristics L. baicalense occupies an intermediate position between subsections Longiunguiculata and Breviunguiculata . There are several interested samples ( L. perenne var. genuinum Krylov, L. perenne var. alpinum (Jacq.) Krylov and L. perenne var. violascens (Bunge) Krylov) in the Krylov Herbarium (Tomsk, TK). It is shown, that these patterns are synonymous with L. perenne , L. altaicum and L. violascens , respectively. The taxonomic significance of some characteristics for Siberian species are discussed. The key for definition of the genus Linum species in flora of Siberia is made. The lectotype of L. stelleroides Planch. are selected. Geographical distribution of species of the genus Linum in the investigated territory is essentially added and corrected.
Independence of G. albifl orum and G. krylovii is proven, new species G. malyschevii Troschkina is described from the East Sayan mountains as the result of a comparative morphological, palynological, chorological analyses of the species. The information about the type material is given, diagnostic features, distribution maps, key for determination to species are provided for all taxa.
The short literary review teratomorphes various taxons of the higher plants is provided. Explicitly teratomorphes of dandelions are considered. The analysis of anomalies of the dandelions arising under the influence of environmentals is given.
The study of microevolution relationships between morphologically close species E. kamczadalorum (Nevski) Tzvelev, E. charkeviczii Probat., and E. kronokensis (Kom.) Tzvelev from Russian Far East was carried out by ISSR-method. The results confirmed the previously obtained data that Kamchatic endemic species E. kamczadalorum is genetically isolated from the complex E. charkeviczii s. l. The latter includes morphotypes with different degrees of expression in diagnostic characters (rachilla’s and lemma’s trichomes). It is widely distributed not only on the Kamchatka Peninsula, but also in continental areas of the Magadan region and, probably, the Republic Sakha (Yakutia). Th e species E. kronokensis which is similar in morphology to E. charkeviczii s. str., but distinctly separated from the latter on ISSR-markers.
Results of the critical revision of Kazakhstanian Boraginaceae collections from the herbaria LE, NS, NSK, TK, KUZ, ALTB (Russia), AA, KG (Kazakhstan), TASH (Uzbekistan) carried out in 1996-2016 are presented. Th e species Myosotis austrosibirica O.D. Nikif. new for the flora of Kazakhstan is recorded. New localities of 18 rare species from genera Gastrocotyle Bunge, Heterocaryum DC., Lappula Gilib., Lepechiniella Popov, Myosotis L. and endemic species Lappula rupestris (Schrenk) Guerke, Lepechiniella saurica (Bajt. et Kudabaeva) Ovczinnikova, Rochelia leiosperma Golosk., according to the botanical-geographical division of Kazakhstan are revealed. Th e reference to the publication of first description and main publications by flora of Kazakhstan are given. Text of original label of new localities with citation of region and place of storage are presented. Information about geographical distribution, diagnostic features and type specimens (if it is necessary) are given in comment.
As a result of specially conducted study seven alien plant species not noted in the Tyva Republic earlier ( Beta vul garis , Cirsium vulgare , Helianthus tuberosus , Lactuca sativa , Oenothera speciosa , Phacelia tanacetifolia , Solidago canadensis ) were revealed. Most of finds are plants escaped from plantings (ergasiophytes). In addition, new localities of eight adventive ( Atriplex hortensis , Elsholtzia ciliata , Galinsoga parvifl ora , Ipomoea purpurea , Lactuca serriola , Matricaria perforata , Saponaria officinalis , Xanthium strumarium ) and three native ( Bolboschoenus planiculmis , Dactylis glomerata , Tripolium vulgare ) species were found. Species names are given in accordance with “Synopsis of Flora of Asian Russia” (2012). Collected dried plant specimens are kept in NS.
